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COLD-PCR Technology


PI Institution IMAT Award Type
Mike Makrigiorgos Dana Faber Cancer Institute R33

COLD-­PCR (COamplification at Lower Denaturation temperature PCR) is a new technology that allows detection of DNA mutations when present in very low copy number ratios. COLD-­PCR preferentially enriches mutant DNA in a mutant/wild-­type mixture by exploitation of the critical temperature, Tc, at which mutation-­containing DNA is preferentially melted over wild-­type.

Microfluidic Genetic Analysis
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COLD-­PCR allows detection of less than one mutant copy per 1,000 wild-­type copies. The technology has multiple applications, including, but not necessarily limited to:

  • Blood-­based cancer mutation testing
  • Definitive genotyping of tumor samples with low percentage malignant cells
  • Mutation detection in circulating tumor cells
  • Identification of resistance clones in primary tumors
  • Very low heteroplasmy detection in mtDNA
  • Viral resistance mutation monitoring

COLD-­PCR promises to provide the enhanced sensitivity and analytical accuracy necessary to screen cancer patients earlier in their individual development of this disease. The technology has been licensed in late 2009/early 2010 by Transgenomic from the Dana Farber Cancer Institute with an exclusive license for Sanger sequencing applications.